No.169, Science (Kexue) Avenue,National HI-TECH Industry Development Zone, Zhengzhou, China [email protected] 0086-371-86549132

ONLINE MESSAGE
MOBILE CEUCHERS

plant transgenic screening:

Reliable method for detection and screening of

2008-12-29 · the screening of transgenic plants that had transformed with a foreign gene. In this report, we demonstrate here that a simple PCR technique can be used to screen putatively transgenic plants transformed with the same endogenous gene. In our laboratory, we have been transformed wheat using one of the high molecular weight glutenin subunit (HMW)

Read More
(PDF) Efficient screening of transgenic plant lines for ...

2021-11-8 · The inheritance-based screening of transgenic plant. lines is always performed via self-pollination and germi-nation of 60 seedlings per plant. In the first

Read More
TaqMan Plant Transgenic Screening NPTII Detection Kit ...

2010-7-19 · 2 TaqMan Plant Transgenic Screening NPTII Detection November 2007 Applied Biosystems The specificity of this method results from the requirement of the enzyme for primer and probe complementarity to target in order for amplification and cleavage to occur. Thus, the fluorescence signal is generated only if the target sequence for

Read More
Efficient screening of transgenic plant lines for ...

2011-4-25 · Efficient screening of transgenic plant lines for ecological research. KLAUS GASE. Department of Molecular Ecology, Max‐Planck‐Institute for Chemical Ecology, Hans‐Knoell‐Strasse 8, 07745 Jena, Germany. Search for more papers by this author. ARNE WEINHOLD.

Read More
A fluorescence-based high-throughput screening method

2020-10-7 · The screening of single-copy transgenic plants. ARR5::eGFP transgenic plants were obtained by screening the seedlings of T1 generation with 1/2 MS media containing 15 µg/mL Hygromycin (Hyg) and 50 µg/mL Timentin (Tim). The single-copy insertion lines were identified by the segregation ratio with 3/4 Hyg resistance of T2 seedlings.

Read More
Timely gene detection assay and reliable screening of ...

2021-4-21 · In our case, the screening of transgenic plants confirmed that both the gfp-ER reporter gene and the npt II selectable marker were integrated into the plant genome. This direct PCR approach provides a powerful tool for large-scale PCR-based gene detection making DNA purification irrelevant.

Read More
(PDF) Basta tolerance as a selectable and screening marker ...

S. von Arnold. Basta tolerance as a selectable and screening marker. for transgenic plants of Norway spruce. Received: 21 October 1999 / Revision received: 24 January

Read More
CSIRO PUBLISHING | Functional Plant Biology

The use of the green fluorescent protein (GFP) and in vivo screening for transgenic cells has enabled the first visual selection of transgenic sugarcane (Saccharum L. hybrid) transformed by Agrobacterium-mediated techniques. Selection of GFP-positive embryogenic callus transformed using Agrobacterium tumefaciensstrain AGL0 was performed by fluorescence microscopy for 6 weeks after ...

Read More
Development of an efficient root transgenic system for ...

2019-2-25 · It offers the possibility of screening a large number of candidate genes more quickly and efficiently. In this work, we first established the root transgenic system for pigeon pea, a woody plant, with a transgenic root induction efficiency of about 39% (Table 1), the ...

Read More
(PDF) Efficient screening of transgenic plant lines for ...

2021-11-8 · The inheritance-based screening of transgenic plant. lines is always performed via self-pollination and germi-nation of 60 seedlings per plant. In the first step, the T. 1.

Read More
Screening for transgenic plant cells that highly express a ...

2002-4-1 · 1.. IntroductionThe production of secondary metabolites, foreign proteins including enzymes, antibodies and antibody fragments, or vaccines in transgenic plant cell suspensions offers broad opportunities for the development of useful products , .Gene manipulation technology is a powerful means for enhancing the production of biologically active compounds, which are generally

Read More
TaqMan Plant Transgenic Screening NPTII Detection Kit ...

2010-7-19 · 2 TaqMan Plant Transgenic Screening NPTII Detection November 2007 Applied Biosystems The specificity of this method results from the requirement of the enzyme for primer and probe complementarity to target in order for amplification and cleavage to occur. Thus, the fluorescence signal is generated only if the target sequence for

Read More
Efficient screening of transgenic plant lines for ...

2011-4-25 · Efficient screening of transgenic plant lines for ecological research. KLAUS GASE. Department of Molecular Ecology, Max‐Planck‐Institute for Chemical Ecology, Hans‐Knoell‐Strasse 8, 07745 Jena, Germany. Search for more papers by this author. ARNE WEINHOLD.

Read More
Fluorescent Screening of Transgenic Arabidopsis Seeds ...

transformation efficiencies necessitate screening pro-cedures for transformed plants employing antibiotic-or herbicide-resistant genes. The selective agents employed in this method of selection quite often exert a negative effect on plant growth, and characterization of the transgenic plants can only be carried out on progenies of later ...

Read More
Kanamycin spray as an effective method of screening ...

2018-8-23 · transgenic plant leaves showed significant chlorotic zones with burnt symptoms. Both the concentrations were differentiating the transgenic BT and nonBT plants but since - kanamycin is a costlier chemical we can go with the lower concentrations.Thus a 1g/L kanamycin solution can be effectively used for screening the cotton plants for true

Read More
(PDF) Basta tolerance as a selectable and screening marker ...

S. von Arnold. Basta tolerance as a selectable and screening marker. for transgenic plants of Norway spruce. Received: 21 October 1999 / Revision received: 24 January 2000 / Accepted: 24 January ...

Read More
Selection of transgenic plants with antibiotics

2017-6-5 · Selection of transgenic plants with antibiotics. 40µg/ml Kanaqmycin, 100µg/ml cefotaxim. Sterilize 200µl (do not exceed) seeds in 1.5 ml tube. Wash 2x with 70% ethanol, remove as much debris as possible. Wash 3 times with autoclaved water. Resuspend in 0.1-0.2

Read More
Production of transgenic tomato plants expressing Cry

2011-12-1 · Transgenic tomato (cv. Money maker) over expressing Bt (Cry 2Ab) gene was produced using Agrobacterium-mediated transformation method.Molecular and biochemical analysis confirmed the expression and integration of the transgene into tomato genome. Obvious effects of Cry 2Ab were judged by the mortality of the American bollworm Helicoverpa armigera (Hübner) and the potato tuber moth

Read More
Enhanced drought tolerance of transgenic rice plants ...

2012-11-7 · Transgenic plants screening transgenicplants were screened GUSstaining polymerasechain reaction (PCR) analysis using primers P1 -TTGCCA AAT CCT ATC ATA CAC-3 -GTCGTC CCA AGT TCC ACA- SWPA2promoter pMnSOD,respectively (Fig

Read More
Screening for transgenic plant cells that highly express a ...

2002-4-1 · 1.. IntroductionThe production of secondary metabolites, foreign proteins including enzymes, antibodies and antibody fragments, or vaccines in transgenic plant cell suspensions offers broad opportunities for the development of useful products , .Gene manipulation technology is a powerful means for enhancing the production of biologically active compounds, which are generally

Read More
Efficient screening of transgenic plant lines for ...

2011-4-25 · Efficient screening of transgenic plant lines for ecological research. KLAUS GASE. Department of Molecular Ecology, Max‐Planck‐Institute for Chemical Ecology, Hans‐Knoell‐Strasse 8, 07745 Jena, Germany. Search for more papers by this author. ARNE WEINHOLD.

Read More
Efficient and rapid method for the screening of transgenic ...

Efficient and rapid method for the screening of transgenic rice plants. Karikalan Jayaraman 1, 2, Amitha Mithra Sevanthi 1, Sivakumar SR 2, Pranab Kumar Mandal 1*. 1 ICAR - National Institute for Plant Biotechnology, Pusa Campus, New Delhi, India. 2 Department of Botany, Bharathidasan University, Tiruchirappalli, Tamil Nadu, India * Corresponding Author: [email protected]

Read More
Transgenic Plants: Gene Constructs, Vector and ...

2018-9-19 · Thus, other screening methods such as polymerase chain reaction (PCR) and reporter gene expression screening are used for better accuracy as an alternative screening method for transgenic plants. Some reporter genes such as the GFP, GUS,

Read More
Transgenic Plants as Low-Cost Platform for ...

Higher plants also represent a suitable system for the risk assessment of chemicals and formulations of human relevance . For example, it has been shown that A. thaliana transgenic lines are applicable for small molecules and drugs screening , but even more rapid and effective approaches may be designed for specific and limited purposes.

Read More
Tissue Culture Methods for the Screening and Analysis of ...

2019-11-6 · duce transgenic plants (4). Putative transgenic plants are usually transplanted from tissue culture to a greenhouse or growth chamber for transgene expression screening (e.g., resistance to pathogens or herbicides, expression of foreign proteins of economic impor-tance, etc.), which often involves testing many lines because trans-

Read More
Transgenic plants and their applications

2013-3-27 · The transgenic plants showed the expression of the luciferase gene by producing light when watered with the substrate luciferin. This reporter gene system provides a simple tool for rapid screening of large numbers of transgenic plants. Points to be considered in all gene transfer programmes 1. Effective systems for the selection of transformed ...

Read More
RNAi-derived transgenic resistance to Mungbean yellow ...

2017-10-27 · Screening of Cowpea (Vigna unguiculata) transgenic plant lines in the T 1 generation for resistance against MYMIV after 5 weeks of Virus infiltration assay. A-C. Appearance of symptoms checked on the topmost leaf of virus challenged plants, D. WT and transgenic plants shown resistance after 35 days of virus challenge.

Read More
Anthocyanin, a novel and user-friendly ... - Plant Methods

2020-7-6 · In the traditional screening methods of transgenic roots based on reporter genes, the β-glucuronidase (GUS) activity or fluorescent proteins (such as GFP, YFP, RFP, CFP, etc.) were the most widely utilized [1, 5,6,7,8,9,10,11,12]. GUS staining assay is destructive to plant tissues, and an expensive chemical substrate (X-Gluc) is required.

Read More
Production of transgenic tomato plants expressing Cry

2011-12-1 · Transgenic tomato (cv. Money maker) over expressing Bt (Cry 2Ab) gene was produced using Agrobacterium-mediated transformation method.Molecular and biochemical analysis confirmed the expression and integration of the transgene into tomato genome. Obvious effects of Cry 2Ab were judged by the mortality of the American bollworm Helicoverpa armigera (Hübner) and the potato tuber moth

Read More
<< Previous:India Hp Cone Crusher Manufacturer
>> Next:Carbonrsquos Golden Malted Waffle Iron

QUALITY SERVICE

Pre-sale service

Shuttle bus

Factory visit

Business tourism

Conference reception

Sale service

On-the-spot investigation

Conceptual design

Detailed quotation

Sign a contract

After-sale service

Packaging delivery

Installation and debugging

Operation training

Regular visit

1.Liming Heavy Industry, a professional manufacturer, can provide perfect quality assurance system for the products.
2.The flexible payment method can help you relieve project stress.
3.Short stocking period and quick delivery speed can reduce project preparation cycle.
4.There are many transportation ways including sea transportation, air transportation, land transportation, which can make your product reach destination quickly.
5.There is a quick response to your problem. And technicians will provide professional assistance at any time.

Copyright © 2004-2015 by China Liming Heavy Industry Science and Technology Co. LTD All rights reserved Industry News Products